Journal: Translational Psychiatry
Article Title: The ventral hippocampus to paraventricular thalamus circuit regulates context-dependent hyperlocomotion through PAC1 receptor signaling in the chronic stress-induced PTSD mouse model
doi: 10.1038/s41398-026-03963-1
Figure Lengend Snippet: (A) The immunofluorescent staining showed that CSDS treatment induced obvious increases in the expression of PAC1 receptor within PVT neurons (upper panel, amplification: 4 x; lower panel, amplification: 60 x). (B) The western blot revealed the apparent increases in the PAC1 receptor within PVT region and (C) the statistical analysis ( n = 5 mice for each group; Mann-Whitney unpaired two-tailed U test; U = 0, ** p = 0.0079). (D) The experimental strategy of cannula injection of PAC1 receptor antagonist and behavioral tests. (E) The context-dependent locomotion activity (One-way ANOVA with Tukey post-hoc test, F(2, 21.47) = 9.201, ** p = 0.0013; Ctrl vs. CSDS, * p = 0.0429; CSDS vs. CSDS + PA-915, *** p = 0.0006). (F) The time spent in open arm (One-way ANOVA with Tukey post-hoc test, F(2, 24.69) = 9.033, ** p = 0.0011; Ctrl vs. CSDS, ** p = 0.0041; CSDS vs. CSDS + PA-915, * p = 0.0163) and (G) number of open arm entries (One-way ANOVA with Tukey post-hoc test, F(2, 26.45) = 6.594, ** p = 0.0047; Ctrl vs. CSDS, * p = 0.0107; CSDS vs. CSDS + PA-915, * p = 0.0193) in the elevated plus maze test. (H) The sociability in the three-chamber test (One-way ANOVA with Tukey post-hoc test, F(2, 17.81) = 6.317, ** p = 0.0084; Ctrl vs. CSDS, * p = 0.0189; CSDS vs. CSDS + PA-915, * p = 0.0173) and (I) the innate motivation in the female encounter test (One-way ANOVA with Tukey post-hoc test, F(2, 22.87) = 7.469, ** p = 0.0032; Ctrl vs. CSDS, * p = 0.0122; CSDS vs. CSDS + PA-915, * p = 0.0102). (J) The arousal levels (One-way ANOVA with Tukey post-hoc test, F(2, 24.94) = 5.412, * p = 0.0112; Ctrl vs. CSDS, * p = 0.044; CSDS vs. CSDS + PA-915, * p = 0.0316). Data was represented as mean ± SD from three independent experiments. n = 10 mice per group per experiment.
Article Snippet: To test the effect of PAC1 receptor agonist and antagonist on the PVT neuronal activity, both PA-915 and maxadilan (Tocris Biosciences) were finally dissolved in ACSF at 5, 20, 50, 100 nM to examine the changes of PVT neuronal firings.
Techniques: Staining, Expressing, Amplification, Western Blot, MANN-WHITNEY, Two Tailed Test, Injection, Activity Assay
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